Introduction：

The EndoFree Maxi Plasmid Plus Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA.  The special Buffer ER and the Spin Columns PM8 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 50 min, ensuring the convenient and quick operation. The plasmid DNA extracted by this kit can be applied to various routine operations, including enzymatic digestion, PCR, sequencing, ligation, transformation as well as  the transfection for various cell types.

Recommended bacteria amount for each practice: For high-copy plasmid, it is recommended to use 100 ml of bacteria culture media; For low-copy plasmid, 200 ml bacteria culture media is recommended.

Features：

1. Unique glass cellulose membrane adsorption technology, efficient and specific binding plasmid DNA;

2. Preparation of Buffer ETR, which can effectively remove more than 99% of endotoxin;

3. The plasmids obtained had good yield and stability.

Storage：

EndoFree Plasmid Maxi Plus Kit can be stored at room temperature (15~25) °C under dry conditions for up to 12 months. Add RNase A to Buffer P1 before first use, mix well and store at 2~8 °C. The mixture can be stored stably for more than 6 months.

Required Reagents：

ethanol

Performance:

High copy plasmid extraction