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FinePure EndoFree Plasmid Maxi Plus Kit

D919

FinePure EndoFree Plasmid Maxi Plus Kit

D919
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Number of views:
1000
Product serial number
D919
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Introduction

The EndoFree Maxi Plasmid Plus Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA.  The special Buffer ER and the Spin Columns PM8 can effectively remove impurities such as endotoxin and protein. The entire extraction process takes only 50 min, ensuring the convenient and quick operation. The plasmid DNA extracted by this kit can be applied to various routine operations, including enzymatic digestion, PCR, sequencing, ligation, transformation as well as  the transfection for various cell types.

 

Recommended bacteria amount for each practice: For high-copy plasmid, it is recommended to use 100 ml of bacteria culture media; For low-copy plasmid, 200 ml bacteria culture media is recommended.

 

Features

1. Unique glass cellulose membrane adsorption technology, efficient and specific binding plasmid DNA;

2. Preparation of Buffer ETR, which can effectively remove more than 99% of endotoxin;

3. The plasmids obtained had good yield and stability.

 

Storage

EndoFree Plasmid Maxi Plus Kit can be stored at room temperature (15~25) °C under dry conditions for up to 12 months. Add RNase A to Buffer P1 before first use, mix well and store at 2~8 °C. The mixture can be stored stably for more than 6 months.

 

Required Reagents

ethanol

 

Performance:

High copy plasmid extraction

 

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Catalog

Specification

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D919

10 preps

1030 RMB

 

 

High-copy plasmid pUC 19, extracted from 150 ml bacterial culture using GENFINE D919 and competitors,was eluted in 1 ml Buffer TB.

Low copy plasmid extraction

Low-copy plasmid pBR322, extracted from 300 ml bacterial culture using GENFINE D919 and competitors, was eluted in 1 ml Buffer TB.

 

 

pEGFP obtained with GENFINE D919 and Competitor T were separately transfected into endotoxin-insensitive cell line 293T and endotoxin-sensitive cell line Huh7 cells by transfection reagent. Expression of GFP were detected in 24 hours after transfection.

 

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2022-05-17 15:57:51
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