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EndoFree Maxi Plasmid Kits

D909

EndoFree Maxi Plasmid Kits

D909
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D909
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The EndoFree Maxi Plasmid Kit adopts a unique silica membrane adsorption technique to efficiently and specifically bind plasmid DNA. By combining the special Endotoxin Removal Buffer P4 and EndoFree Maxi Filtration Columns, endotoxin, proteins and other impurities can be effectively removed. The whole extraction process takes only 1 hour, ensuring the convenient and quick operation. The plasmid DNA extracted by this kit can be applied to various routine operations, including enzymatic digestion, PCR, sequencing, ligation, transformation as well as  the transfection for various cell types.

Recommended bacteria amount for each practice: For high-copy plasmid, it is recommended to  use 100 ml of bacteria culture media, and the yield from which is generally around 500-1500 μg. For low-copy plasmid, 200 ml bacteria culture media is recommended to generate around 200-600 μg plasmids.

 

 

Features

  Fast and high-yield: 200 μg-1.5 mg plasmids can be extracted in about 1 hour, and there are many supercoiled plasmids.

  Efficient transfection: suitable for advanced transfection experiments of most cell lines.

  Wide range of applications: suitable for routine experiments such as restriction digestion, PCR, sequencing, connection, transformation, and gene therapy,High-end experiments such as cell microinjection, gene silencing, and transfection.

 

Storage

EndoFree Maxi Plasmid Kits can be stored at room temperature (15-25°C) under dry conditions for up to 12 months. Add RNase A to Buffer P1 before the first use, mix well and store at 2-8°C. The mix can be stored stably for more than 6 months.

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D909

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