This kit is based on centrifugal adsorption column technology and is suitable for the recovery of 50bp-30Kb DNA fragments from agarose gels at various concentrations. In addition, the kit is also suitable for the recovery and purification of DNA from PCR products, enzymatic reaction solutions, or from crude DNA (including genomic DNA) obtained by various methods. Buffer PB contains pH indicator and the solution is yellow, which is convenient to determine whether the pH value of the solution is suitable for binding with DNA adsorption column. The efficiency of DNA recovery can be up to 80%, and the purified DNA can be directly used for sequencing, ligation, digestion, PCR, labeling, etc.

Features

◈  Strong compatibility: It can be used for direct purification of DNA products, and it can also be used for DNA gel recovery.

◈  Easy to operate, large volume of recyclable glue: it can be sol according to the ratio of glue block and sol liquid.

◈  Stable and reliable: Equipped with an indicator, it can intuitively judge the pH value that affects DNA adsorption, and it can also judge whether the DNA is fully combined with the membrane and improve the recovery efficiency.

Storage

The kit can be stored at room temperature (15-25°C) under dry conditions for 12 months. There may be precipitation in Buffer PB at low temperature. Redissolve it in 37 ℃ water bath and shake well before use.