Sensitivity 

Figure 1：According to WHO International Standard 3rd WHO International

Standard for Hepatitis B Virus for Nucleic Acid Amplification technology NIBSC code:10/264 ,dilute virus to 5 IU/mL; The experiment was set to repeat for 30 times，quantitative detection of hepatitis B virus nucleic acid ( based-on PCR-Fluorescent probe method) was used as an amplification reagent to detect the detection rate，The repeat detection rate is100%（30 out of 30）.

Linearity

Figure 2：High load HBV-positive samples, which are calibrated according to WHO International Standard 3rd WHO International Standard for Hepatitis B Virus for Nucleic Acid Amplification technology NIBSC code:10/264 , are diluted to 8 gradients （5.0 × 109 IU/mL～5.0 × 102 IU/mL）； Hepatitis B virus nucleic acid was subjected to Real Time Quantitative PCR (PCR-fluorescent probe method), three replicates at each concentration, results as above.