The pandemic has influenced the world extensively, especially in the biotech industry. Many IVD companies have found that the supply chain has become much more expensive and unstable compared to the pre-pandemic era. Therefore, finding alternative suppliers has become one of the most effective ways to increase supply chain stability.
Nanotein is a subsidiary of the Genfine Group. Suzhou Nanotein Biotechnology Co., Ltd. is committed to the corporate culture of ‘customer-centric, technological innovation-oriented, and provides stable, reliable, cost-effective independent products and professional integrated solutions for the field of life sciences. Provide high-quality microsphere materials with precise and controllable size, material, structure and matrix for biomedicine, blood products, diagnosis, detection, and other fields, and strive to become the world's leading supplier of biological microsphere materials and services.
Core Materials for Nucleic Acids Extraction- Magnetic Beads
Silica-coated Hydroxyl Beads
This is the most widely used type of magnetic bead When extracting nucleic acids using silica-coated hydroxyl beads high concentrations of chaotropic salts (Nal, NaClO4, GuHCl, GuSCN, etc.) are usually required. The high concentration of salt ions can effectively shield the electrostatic repulsion in the solution. At the same time, chaotropic salt ions can also competitively bind to the surface of magnetic beads and nucleic acid molecules, destroy the original hydration layer on the surfaces of the two and promote the adsorption of nucleic acid molecules on the surface of magnetic beads (hydrogen bond + van der Waals force). Since commonly used chaotropic salts such as guanidine ions inhibit PCR, they must be removed in subsequent washing steps, and the removal can be monitored by absorbance ratio.
The surface of Nanotein Silica-coated Hydroxyl Magnetic Beads is modified with hydroxyl groups, which has good nucleic acid extraction performance, good dispersibility and short reaction time, and is suitable for most nucleic acid extraction systems and extractors on the market and has excellent extraction performance for various complex samples.
● Strong adaptability
● Applicable to most extraction systems, under various complex samples
● Fast magnetic response
● Reduce extraction time and improve extraction efficiency
● Stable performance
● High consistency between batches, long validity period and stable performance for various samples
◆ Carboxy Magnetic Beads
Carboxyl magnetic beads can bind nucleic acid molecules in a high concentration chaotropic salt environment like Silica-coated Hydroxyl Magnetic Beads. In addition, they can also bind to nucleic acid molecules through another special mechanism. By adding a certain concentration of PEG and NaCl to the solution, the nucleic acid molecules can be gradually rolled up from the extended conformation into small balls, and most of the negative charges on them are shielded, which promotes the adsorption of nucleic acid molecules to the magnetic beads. The larger the molecular weight of the nucleic acid molecule, the more prone it is to undergo this conformational change from a stretched coil to a curled ball. Therefore, by adjusting the volume ratio of the salt solution to the nucleic acid sample, the preferential adsorption of nucleic acid fragments with larger molecular weights on the surface of the carboxyl magnetic beads can be achieved to achieve the so-called fragment screening effect.
Case 1 MS01H Performance in Company S’s Buffer
Conclusion: The extraction effect of MS01H magnetic beads in the Company S buffer system is 2 Ct values earlier than that of Company S own magnetic beads.
Case 2: MS01H Performance in Company D’s Buffer
Conclusion: The extraction effect of MS01H magnetic beads in the Company D buffer system is 1-2 Ct values earlier than that of Company D’s own magnetic beads.
Case 3 MS01H Performance in Company S2’s Buffer
Conclusion: The extraction effect of MS01H magnetic beads in the Company S2 buffer system is equal to Company S2 own magnetic beads.
Case 4 MS01H Performance in Company J’s Buffer
Conclusion: The extraction effect of MS01H magnetic beads in the Company S2 buffer system is equal to Company J’s own magnetic beads.
Case 5 Thermal accelerated stability test
(1) The extraction effect of different batches of MS01H magnetic beads in the Company J buffer system is equivalent to that of its own magnetic beads.
(2) At low concentrations, MS01H magnetic beads have better sensitivity.
(3) After the magnetic beads were heated under 55°C for 2 months, there was no difference between the extraction performance and the performance of the magnetic beads stored in cold storage.
Case 6 MS02H Performance in Company M’s Buffer
Conclusion: The extraction effect of MS02H magnetic beads in the Company M buffer system is equal to Company M’s own magnetic beads.
Case 7: MS01H and MS01C’s performance in Company A’s buffer
Conclusion: The extraction effect of MS01H and MS01C magnetic beads in the Company A buffer system is equal to Company A own magnetic beads.
Case 8 MS03H Extraction Effect in Whole Blood Genome Extraction Reagent Buffer
Conclusion: The genome was extracted from human whole blood samples, the OD260/230 of the genome extracted by MS03H magnetic beads were higher than 1.80.
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