The epidemic has promoted the development of the nucleic acid extraction industry. An increasing number of nucleic acid extraction reagents, instruments and integrated solutions have been put into the market, which has improved sample throughput and shortened experimental time and human resources However, the qualities of the reagents of different manufacturers are different. Therefore, the extracted templates may have different residual impurities. The residual impurities will directly affect the downstream fluorescence quantitative experiment. Ineffective amplification may occur. To fundamentally solve this problem, Genfine has launched a super anti-reverse enzyme, which is compatible with a variety of complex templates.
Introducing: Flash Robust HotStart DNA Polymerase.
Flash Robust HotStart DNA Polymerase is a new type of hot-start polymerase based on wild-type Taq Pol by directed transformation. It has strong resistance to blood and PCR inhibitors. Using double-antibody blocking, it has ultra-high amplification efficiency, good amplification sensitivity and specificity. Heated at the pre-denaturation temperature for 30 seconds, the blocking antibody can be completely inactivated, and the DNA polymerase activity is released.
Flash Robust HotStart DNA Polymerase has ultra-high amplification efficiency, ultra-high resistance, and high sensitivity, and is compatible with the amplification of primers and probes with different GC.
1. Super high resistance
Resistant to a variety of high-concentration inhibitors, such as EDTA, guanidine hydrochloride, heparin, sodium acetate, etc. It can be used for direct blood expansion and can effectively expand 20% of whole blood samples.
1.1 Comparison experiment between A210 and competing blood direct expansion
ASF-positive blood was used for direct blood expansion, and the results were as follows:
▲ASF-positive blood is directly expanded, A210 can amplify 20% of blood samples, and competing products have no amplification.
1.2 A210 is used for plasma direct expansion, plasma ratio comparison experiment
▲In the plasma direct expansion qPCR system, A210 can amplify 30% of plasma.
2. High sensitivity
The input amount of ordinary PCR samples are as low as 400pg, and qPCR can detect single-digit copy number samples.
2.1 Test 9 different brands of HS Taq for Q-PCR detection, where E is Genfine A210 Flash Robust HotStart DNA Polymerase.
▲The sensitivity of Genfine A210 is better than that of most competing products.
3. Compatible with the amplification of primer probes with different GC contents
3.1 Comparison of the amplification effects of A210 and competing products on primer probes with different GC contents
Using the human genome as a template, primers and probes with different GC contents were used to verify the performance of A210. The results are as follows:
▲The results show that Genfine A210 has better compatibility for primer probes with different GC contents.
4. Rapid expansion
The amplification speed of complex template amplification within 3kb can reach 5-15sec/kb, and the amplification speed of simple templates can reach 1sec/kb.
▲When the extension time is 1s/kb, the linearity of the amplification curve of Genfine A210 is better than that of competing products.
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